Composite

Part:BBa_K3747137:Design

Designed by: Sophieke Lems   Group: iGEM21_Wageningen_UR   (2021-10-21)


napEDABC (Periplasmic nitrate reductase) Pseudomonas stutzeri JM300


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 852
    Illegal EcoRI site found at 2205
    Illegal SpeI site found at 2156
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 852
    Illegal EcoRI site found at 2205
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal SpeI site found at 2156
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 852
    Illegal EcoRI site found at 2205
    Illegal BglII site found at 1846
    Illegal BglII site found at 3859
    Illegal XhoI site found at 411
    Illegal XhoI site found at 1527
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 852
    Illegal EcoRI site found at 2205
    Illegal SpeI site found at 2156
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 852
    Illegal EcoRI site found at 2205
    Illegal SpeI site found at 2156
    Illegal NgoMIV site found at 219
    Illegal NgoMIV site found at 1201
    Illegal NgoMIV site found at 1971
    Illegal NgoMIV site found at 2818
    Illegal NgoMIV site found at 3121
    Illegal NgoMIV site found at 3627
    Illegal AgeI site found at 663
    Illegal AgeI site found at 2667
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Source

Pseudomonas stutzeri JM300

References